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KMID : 1171820060210010052
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Journal of Environmental and Sanitary Engineering
2006 Volume.21 No. 1 p.52 ~ p.57
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Identification of the Antioxidative Function of Metallothionein by Oxidation of NADPH and Production of Nitrite
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Kim Kwan-Chun
Kim Joon-Tae
Kim Hee-Joung
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Abstract
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Metallothioneins(MTs) belong to the class of low molecular weight proteins. Recently, it has been suggested that MTs may play a direct role in cellular defense against oxidative stress by functioning as antioxidants. Oxidative damage to different cellular components makes a major contribution to many pathogenenesses.Several studies have demonstrated that MT is able to quench a wide range of reactive oxygen species at a higher efficiency than other well known antioxidants such as superoxide dismutate(SOD). The present study was designed to evaluate the effect of MT on the activities of the reactive oxygen species removal system. MT showed the scavenging of superoxide in the SOD assay system in the presence or absence of SOD. When MT was added to nicotinamide adenine dinucleotide phosphate(NADPH) oxidation system in presence of fixed amount of SOD increase the breakdown rate of superoxide. When MT was added to the system that form nitrite from hydroxylammonium chloride, the formation of nitrite was inhibit. We concluded that the function of MT as antioxidant might have an effect on the level of superoxide scavenging.
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KEYWORD
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metallothionein(MT), antioxidants, superoxide dismutate(SOD), nicotinamide adenine dinucleotide phosphate(NADPH)
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